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Go to this link. Firstly, the unlabeled primary antibody is incubated with the sample containing the antigen of interest, leading to the formation of antigen-antibody complex Ag-Ab. Finally, Click the Save Button. In this way, the target protein within cells is detected.
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Needs the use of inhibitor antigen. I have noticed some of the variations that Geoff Davis describes, most of the lasses in my office used the "statement that sounds like a question" delivery which took some getting used to.
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